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Augustine et al. (2011) Plant Cell. 23:3696-3710

Actin interacting protein 1 and depolymerizing factor drive rapid actin dynamics

Supplemental Movie 1. Dynamic behavior of cortical F-actin in a representative LA cell. Images were collected with a variable angle epifluorescence microscope at 30-ms intervals. Images were adjusted by background subtraction, enhanced contrast, and smoothing in ImageJ. Movie plays back 33.3 fps. Stills from this movie are shown in Figure 7A.
Supplemental Movie 2. Dynamic behavior of cortical F-actin in a representative NLS4 LA cell. Images were collected with a variable angle epifluorescence microscope at 30-ms intervals. Images were adjusted by background subtraction, enhanced contrast, and smoothing in ImageJ. Movie plays back 33.3 fps. Stills from this movie are shown in Figure 7A.
Supplemental Movie 3. Dynamic behavior of cortical F-actin in a representative NLS4 LA GUS-RNAi cell. Images were collected with a spinning disc confocal microscope at 616-ms intervals. Images were adjusted by background subtraction, enhanced contrast, and smoothing in ImageJ. Movie plays back 1.6 fps. Stills from this movie are shown in Figure 7A.
Supplemental Movie 4. Dynamic behavior of cortical F-actin in a representative NLS4 LA ∆aip1 cell. Images were collected with a variable angle epifluorescence microscope at 30-ms intervals. Images were adjusted by background subtraction, enhanced contrast, and smoothing in ImageJ. Movie plays back 33.3 fps. Stills from this movie are shown in Figure 7A.
Supplemental Movie 5. Dynamic behavior of cortical F-actin in a representative NLS4 LA ADF-RNAi cell. Images were collected with a spinning disc confocal microscope at 616-ms intervals. Images were adjusted by background subtraction, enhanced contrast, and smoothing in ImageJ. Movie plays back 1.6 fps. Stills from this movie are shown in Figure 7A.
Supplemental Movie 6. An example of cortical F-actin translocation in a representative LA cell. (left) Movie is shown without any markings. (right) Filament that undergoes the translocation event is marked in red. Images were collected with a variable angle epifluorescence microscope at 60-ms intervals. Images were adjusted by background subtraction, enhanced contrast, and smoothing in ImageJ. Movie plays back 16.6 fps. Stills from this movie are shown in Figure 8A.
Supplemental Movie 7. An example of cortical F-actin translocation in a representative NLS4 LA ∆aip1 cell. (left) Movie is shown without any markings. (right) Filament that undergoes the translocation event is marked in red. Images were collected with a variable angle epifluorescence microscope at 60-ms intervals. Images were adjusted by background subtraction, enhanced contrast, and smoothing in ImageJ. Movie plays back 16.6 fps. Stills from this movie are shown in Figure 8A.
Supplemental Movie 8. An example of cortical F-actin rearrangements around a bundled cluster of filaments in a representative LA cell. (left) Movie is shown without any markings. (right) Marked in red is a filament that depolymerizes. Marked in green is a filament that grows out of the bundled center. Images were collected with a variable angle epifluorescence microscope at 60-ms intervals. Images were adjusted by background subtraction, enhanced contrast, and smoothing in ImageJ. Movie plays back 16.6 fps. Stills from this movie are shown in Figure 8B.
Supplemental Movie 9. An example of cortical F-actin rearrangements around a bundled cluster of filaments in a representative NLS4 LA ∆aip1 cell. Images were collected with a variable angle epifluorescence microscope at 60-ms intervals. Images were adjusted by background subtraction, enhanced contrast, and smoothing in ImageJ. Movie plays back 16.6 fps. Stills from this movie are shown in Figure 8B.
Supplemental Movie 10. An example of a severing event in a representative LA cell. (left) Movie is shown without any markings. (right) Filament that is severed is marked in red. Images were collected with a variable angle epifluorescence microscope at 60-ms intervals. Images were adjusted by background subtraction, enhanced contrast, and smoothing in ImageJ. Movie plays back 5 fps. Stills from this movie are shown in Figure 8C.
Supplemental Movie 11. An example of a severing event in a representative NLS4 LA ∆aip1 cell. (left) Movie is shown without any markings. (right) Filament that is severed is marked in red. Images were collected with a variable angle epifluorescence microscope at 60-ms intervals. Images were adjusted by background subtraction, enhanced contrast, and smoothing in ImageJ. Movie plays back 5 fps. Stills from this movie are shown in Figure 8C.

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