| Supplemental Movie 1. Dynamic behavior of cortical F-actin in a representative LA cell. Images were collected with a variable angle epifluorescence microscope at 30-ms intervals. Images were adjusted by background subtraction, enhanced contrast, and smoothing in ImageJ. Movie plays back 33.3 fps. Stills from this movie are shown in Figure 7A.
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| Supplemental Movie 2. Dynamic behavior of cortical F-actin in a representative NLS4 LA cell. Images were collected with a variable angle epifluorescence microscope at 30-ms intervals. Images were adjusted by background subtraction, enhanced contrast, and smoothing in ImageJ. Movie plays back 33.3 fps. Stills from this movie are shown in Figure 7A. |
| Supplemental Movie 3. Dynamic behavior of cortical F-actin in a representative NLS4 LA GUS-RNAi cell. Images were collected with a spinning disc confocal microscope at 616-ms intervals. Images were adjusted by background subtraction, enhanced contrast, and smoothing in ImageJ. Movie plays back 1.6 fps. Stills from this movie are shown in Figure 7A. |
| Supplemental Movie 4. Dynamic behavior of cortical F-actin in a representative NLS4 LA ∆aip1 cell. Images were collected with a variable angle epifluorescence microscope at 30-ms intervals. Images were adjusted by background subtraction, enhanced contrast, and smoothing in ImageJ. Movie plays back 33.3 fps. Stills from this movie are shown in Figure 7A. |
| Supplemental Movie 5. Dynamic behavior of cortical F-actin in a representative NLS4 LA ADF-RNAi cell. Images were collected with a spinning disc confocal microscope at 616-ms intervals. Images were adjusted by background subtraction, enhanced contrast, and smoothing in ImageJ. Movie plays back 1.6 fps. Stills from this movie are shown in Figure 7A.
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| Supplemental Movie 6. An example of cortical F-actin translocation in a representative LA cell. (left) Movie is shown without any markings. (right) Filament that undergoes the translocation event is marked in red. Images were collected with a variable angle epifluorescence microscope at 60-ms intervals. Images were adjusted by background subtraction, enhanced contrast, and smoothing in ImageJ. Movie plays back 16.6 fps. Stills from this movie are shown in Figure 8A. |
| Supplemental Movie 7. An example of cortical F-actin translocation in a representative NLS4 LA ∆aip1 cell. (left) Movie is shown without any markings. (right) Filament that undergoes the translocation event is marked in red. Images were collected with a variable angle epifluorescence microscope at 60-ms intervals. Images were adjusted by background subtraction, enhanced contrast, and smoothing in ImageJ. Movie plays back 16.6 fps. Stills from this movie are shown in Figure 8A. |
| Supplemental Movie 8. An example of cortical F-actin rearrangements around a bundled cluster of filaments in a representative LA cell. (left) Movie is shown without any markings. (right) Marked in red is a filament that depolymerizes. Marked in green is a filament that grows out of the bundled center. Images were collected with a variable angle epifluorescence microscope at 60-ms intervals. Images were adjusted by background subtraction, enhanced contrast, and smoothing in ImageJ. Movie plays back 16.6 fps. Stills from this movie are shown in Figure 8B. |
| Supplemental Movie 9. An example of cortical F-actin rearrangements around a bundled cluster of filaments in a representative NLS4 LA ∆aip1 cell. Images were collected with a variable angle epifluorescence microscope at 60-ms intervals. Images were adjusted by background subtraction, enhanced contrast, and smoothing in ImageJ. Movie plays back 16.6 fps. Stills from this movie are shown in Figure 8B.
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| Supplemental Movie 10. An example of a severing event in a representative LA cell. (left) Movie is shown without any markings. (right) Filament that is severed is marked in red. Images were collected with a variable angle epifluorescence microscope at 60-ms intervals. Images were adjusted by background subtraction, enhanced contrast, and smoothing in ImageJ. Movie plays back 5 fps. Stills from this movie are shown in Figure 8C.
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| Supplemental Movie 11. An example of a severing event in a representative NLS4 LA ∆aip1 cell. (left) Movie is shown without any markings. (right) Filament that is severed is marked in red. Images were collected with a variable angle epifluorescence microscope at 60-ms intervals. Images were adjusted by background subtraction, enhanced contrast, and smoothing in ImageJ. Movie plays back 5 fps. Stills from this movie are shown in Figure 8C. |