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McKenna et al. (2009) Plant Cell. 21: 3026-3040

Exocytosis preceds and predicts the increase in growth in oscillating pollen tubes.

Supplemental Movie 1 DIC image of lily pollen tube showing oscillating wall thickness. The movie shows the transition between the wide and thin spacing between the two fringes. Images collected at 1 second intervals.
Supplemental Movie 2 PI staining of lily pollen tube. This movie shows a lily pollen tube stained with PI. The brightest fluorescence occurs at the cell apex and oscillates in intensity along with growth. Images acquired at 3 second intervals. The image is pseudocolored with red indicating high fluorescence and blue indicating low.
Supplemental Movie 3 PI staining of tobacco pollen tube. This movie shows a tobacco pollen tube stained with PI. The brightest fluorescence occurs at the cell apex and oscillates in intensity along with growth. Images acquired at 5 second intervals. The image is pseudocolored with red indicating high fluorescence and blue indicating low.
Supplemental Movie 4 Tobacco pollen tube expressing PME-GFP. This movie shows the change in PME-GFP fluorescence during growth.

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