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Vidali et al. (2009) PLoS One. 4:e5744

Lifeact-mEGFP reveals a dynamic apical F-actin network in tip growing plant cells.

Movie S1. Time lapse LSCM of Lifeact-mEGFP in a moss caulonemal cell. Two frames were acquired per second. Total elapsed time was 179.5 seconds. Scale bar is 5 µm.

Movie S2. Time lapse LSCM of Nicotiana tobaccum pollen tube transformed with Lifeact-mEGFP. One frame was acquired every five seconds. Total elapsed time was 245 seconds. Scale bar is 10 µm.

Movie S3. Time lapse LSCM of Lilium formosanum pollen tube transformed with Lifeact-mEGFP. One frame was acquired every three seconds. Total elapsed time was 96 seconds. Scale bar is 10 µm.

Movie S4. Time lapse spinning disc confocal images of Lifeact-mEGFP expressing Moss caulonemal cell. One frame was acquired every two seconds. Total elapsed time was 120 seconds.

Movie S5. Time lapse LSCM DIC images of Lilium formosanum pollen tube transformed with Lifeact-mEGFP and treated with 2 nm Latrunculin B. One frame was acquired every three seconds. Total elapsed time was 246 seconds. Scale bar is 10 µm.

Movie S6. Time lapse LSCM fluorescence images of Lilium formosanum pollen tube transformed with Lifeact-mEGFP and treated with 2 nm Latrunculin B. One frame was acquired every three seconds. Total elapsed time was 246 seconds. Scale bar is 10 µm.


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