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Vidali et al. (2010) Plant Cell. 22:1868-1882

Myosin XI is essential for tip growth.

Supplemental Movie 1. Dynamic behavior of cortical F-actin in a representative control cell. Images were collected with a laser scanning confocal microscope at 2-s intervals. Images were contrast enhanced with the equalized histogram and smooth function from ImageJ.
Supplemental Movie 2. Dynamic behavior of cortical F-actin in a representative myosin XI-RNAi cell. Images were collected with a laser scanning confocal microscope at 2-s intervals. Images were contrast enhanced with the equalized histogram and smooth function from ImageJ.

Supplemental Movie 3. Dynamic behavior of cortical F-actin in a representative wildtype cell expressing Lifeact-mEGFP treated with DMSO. Images were collected with a laser scanning confocal microscope at 2-s intervals. Images were contrast enhanced with the equalized histogram and smooth function from ImageJ.

Supplemental Movie 4. Dynamic behavior of cortical F-actin in representative wild-type cell expressing Lifeact-mEGFP treated with 20 µM Jasplakinolide. Images were collected with a laser scanning confocal microscope at 2-s intervals. Images were contrast enhanced with the equalized histogram and smooth function from ImageJ.

Supplemental Movie 5. Localization of 3xmEGFP-myosin XIa in a growing caulonemal cell. Images are the maximum projection of five images collected at 1-µm intervals every 5 s. Images were acquired with a laser scanning confocal microscope. Note the transient accumulation of 3xmEGFP-myosin XIa at the cell apex.

Supplemental Movie 6. Localization of 3xmEGFP-myoXIa in an emerging branch cell. Images are single focal planes collected every 5 s. Images were acquired with a laser scanning confocal microscope.

Supplemental Movie 7. Localization of 3xmEGFP-myoXIa in a growing chloronemal cell. Images are single focal planes collected every 5 s. Images were acquired with a laser scanning confocal microscope.

Supplemental Movie 8. Localization of Lifeact-mEGFP in a growing caulonemal cell. Images are the maximum projection of five images collected at 1-µm intervals every 5 s. Images were acquired with a laser scanning confocal microscope. Note the transient accumulation of F-actin at the cell apex, which is similar to that of 3xmEGFP-myosin XIa.

Supplemental Movie 9. Localization of mEGFP in a growing protonemall cell. Images are the maximum projection of five images collected at 1-µm intervals every 5 s. Images were acquired with a laser scanning confocal microscope. Note that mEGFP does not accumulate at the cell apex, which supports the specific accumulation of 3xmEGFP-myosin XIa in this region.

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